Endocrine Health Foundation

AROMATASE HIGH THROUGHPUT SCREENING for REPRODUCTIVE TOXICITY: see our publications and presentations on drug interactions with estrogen endocrinology

  • Kragie L, Turner SD, Patten CJ, Crespi CL, Stresser DM. 2002 Assessing pregnancy risks of azole antifungals using a high throughput aromatase inhibition assay. Endocrine Research 28 (3): 129-140
  • Kragie L. 2002 Aromatase in primate pregnancy: a review. Endocrine Research 28 (3): 121-128
  • Stresser DM, Turner SD, Ho T, Crespi CL, Kragie L. Aromatase Inhibition by Azole Drugs: Assessing Pregnancy Risks. Society of Toxicology Annual Meeting, Nashville TN, March 2002
  • Kragie L, Stresser DM, Turner SD, Patten CJ, Crespi CL. Assessing pregnancy risks using a high throughput aromatase inhibition assay. ASCPT Annual Meeting, Atlanta GA, March 2002. [Abstract #TPII-94] Clinical Pharmacology & Therapeutics Feb 2002; 71(2): P67
  • Kragie L. EPA Endocrine Disruptor Tier 1 Screening: Peer Review Results for Aromatase EPA publication 2008

ASCPT Workshop Orlando FL 2005, "Interaction of Drugs with Estrogen Endocrinology" [pictured above]

  • Laura Kragie MD, "Introduction and Background"
  • Jeffrey Dodge PhD, "An Illustrative Example of the Identification of a New SERM Targeted to Affect the Hypothalamic-Pituitary-Ovarian Axis"
  • David Stresser PhD, "High Throughput Testing for Inhibition of p450s Including Aromatase (CYP19) and Overview of the EPA's Endocrine Disruptor Screening Program"
  • David Flockhart MD PhD, "Overview of Estrogen and p450 Drug Metabolism, Drug Interactions, and Pharmacogenomics of Aromatase"
  • Eugene Albrecht PhD, "Role of Estrogen and Aromatase in Pregnancy and Fetal Gonadal Development"
  • LEARNING OBJECTIVES: Understand the process of tissue generated estrogen from circulating androgen precursors/prohormones and consequences of inhibiting the in situ production of estrogen, in men, women, fetus and child. Review in vitro screening tools for endocrine disruptors of estrogenic production/receptor cascade, and the EPAs Endocrine Disruptor Screening Testing Advisory Committee (EDSTAC) guidelines. Examine the interaction of drugs with the Hypothalamic -Pituitary-Ovarian (HPO) axis and estrogen cascade as illustrated by the history of a new Selective Estrogen Modulator (SERM) targeted to treat gynecologic conditions.
  • Seminar Merck Pharmaceuticals, Wayne NJ, "Azole Aromatase Inhibitors and their Clinical Implications" 2005: Laura Kragie MD

THYROID HORMONES INTERACTION with MEMBRANE DRUG TRANSPORTERS: see our publications and presentations on drug interactions with thyroid endocrinology

Original Reports/Peer Reviewed Journals:

  • Kragie L, Doyle D. 1992 Benzodiazepines inhibit temperature-dependent [125]I-L-triiodo-thyronine accumulation into human liver, human neuroblast and rat pituitary cell lines. Endocrinology 130: 1211-1216
  • Kragie L. 1992 Requisite structural characteristics for benzodiazepine inhibition of triiodothyronine uptake into a human liver cell line. Life Sciences, Pharmacology Letters 51: PL83-PL88
  • Kragie L. 1993 Neuropsychiatric implications of thyroid hormone-benzodiazepine interactions. Endocrine Research 19: 1-32
  • Kragie L, Kwon YW, Smiehorowski R. 1993 Rat cardiac calcium channels and their relationships with b-adrenergic and muscarinic receptors in hypothyroidism. Endocrine Research19: 57-71
  • Kragie L, Smiehorowski R. 1993 Measures of skeletal muscle calcium channels and acetylcholine receptors in thyroidectomized rats. Endocrine Research 19: 207-219
  • Kragie L, Stock ME. 1994 Acetylcholinesterase activity in fast-twitch skeletal muscle from thyroidectomized rats. Endocrine Research 20: 47-57
  • Kragie L, Forrester ML, Cody V, McCourt M. 1994 Computer-assisted molecular modeling of benzodiazepine and thyromimetic inhibitors of the HepG2 iodothyronine transporter. Molecular Endocrinology 8: 382-391
  • Kragie L, Smiehorowski R. 1994 Altered peripheral benzodiazepine receptor binding in cardiac and liver tissues from thyroidectomized rats. Life Sciences 55 (24): 1911-1918
  • Kragie L. 1994 Membrane iodothyronine transporters part I: review of physiology. Endocrine Research 20 (4): 319-342
  • Kragie L. 1996 Membrane iodothyronine transporters part II: review of protein biochemistry. Endocrine Research 22 (2):95-119

Abstracts of Presentations at Scientific Society Meetings:

  • Kragie L, Doyle D. Benzodiazepines inhibit triiodothyronine transport into human liver cell line. Society for Neuroscience Annual Meeting, 1990 [Program Abstract #36.19]
  • Kragie L, Doyle D. Benzodiazepine inhibition of triiodothyronine uptake into human liver cell line has a unique structure activity relationship. Society for Neuroscience Annual Mtg, 1991 [Abstract #537.10]
  • Kragie L, Forrester ML, Cody V. Structural characteristics of benzodiazepine compounds which inhibit triiodothyronine cellular uptake. The 74th Annual Endocrine Society Mtg, 1992 [Abstract #428]
  • Kragie L, Forrester ML, Cody V. Comparison of benzodiazepine inhibitors of thyroid hormone cellular uptake to thyroid hormones, using crystallographic computer modeling. 23rd National Medicinal Chemistry Symposium, Division American Chemical Society, 1992 [Abstract]
  • Kragie L, Smiehorowski R. Peripheral benzodiazepine receptor binding in hypothyroid rat ventricles. Society for Neuroscience Annual Meeting, 1992 [Abstract #486.15]
  • Kragie L, Smiehorowski R, Goonawardena D. Putative iodothyronine transporter membrane proteins from liver and brain as identified with antibodies and ligand affinity chromatography. The 75th Annual Endocrine Society Mtg, 1993 [Abstract #818]
  • Lui Y, Kragie L, Smiehorowski R, Marfella C, Lakshmanan M. Polyclonal antibodies to benzodiazepine binding proteins decrease triiodothyronine uptake into mouse neuroblasts. American Federation for Clinical Research Mtg, 1994 [Abstract]
  • Lakshmanan M, McCourt M, Lui Y, Kragie L. Thyroid hormone plasma membrane transport: structural homology and binding site interactions with aminoacids and benzodiazepines. The American Thyroid Association Annual Mtg, 1994. [Abstract]
  • Kragie L, McCourt M, Lakshmanan M. Modeling the iodothyronine and aminoacid transport sites in mouse neuroblastoma cells. 77th Annual Endocrine Society Mtg, 1995 [Abstract #P3-422] p 574